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Immunostaining After organotypic culture or cryosection, the cochlear explants or tissue sections were fixed with 4% PFA, permeabilized with 1% TritonX-100 in PBS, and blocked by incubation in PBT-1 solution (0.1% Triton X-100, 8% donkey serum, 1% bovine serum albumin, and 0.02% sodium azide in PBS) at room temperature for 1 h

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Moreover, significant changes in microbial communities combined with different dietary compositions may drive different molecular inputs to neuroendocrine signaling pathways, and these altered inputs (e.g., short-chain fatty acid response) combined with changes in the gut-brain axis may drive different systemic responses at the level of hormones and gene expression (49)